To begin, you must ensure your data is in the correct format. Prism requires actual counts —meaning the raw number of individuals, events, or items. Mutual Exclusivity : Each subject must contribute to exactly one cell only. No Percentages

Data Entry

To ensure your results are "verified" by the software, follow the standard workflow in GraphPad Prism : : Enter your data into a Contingency table .

Suppose we want to determine if there is an association between the type of treatment and the outcome of a disease. We collect the following data:

To ensure your GraphPad Chi-Square analysis is verified and ready for presentation:

• $a=45, b=30, c=10, d=25$
• Total $N = 110$

Enter Raw Counts

: Input your data into the grid where rows represent groups (e.g., treatment) and columns represent outcomes (e.g., pass/fail). Do not use normalized values .

Step 4: Run the Test

After clicking OK on the parameters dialog, Prism will generate a "Results" sheet. Here is how to read the key values: